Research Fellow - Lab Medicine and Pathology

The candidate will be working under the mentorship of Dr. Maia and Dr. Harrington in a joint project to define transcriptional dependencies associated with serous (p53 Aberrant) endometrial cancer.

Responsibilities:

• Cultivate mammalian cell cultures, primarily intestinal organoid/tumoroid models
• Derive and establish normal and cancer organoid lines frompatient samples
• Design and conduct drug screenings and evaluate the efficacy of novel therapeutics in preclinical organoid models.
• Use reporter expressing organoid models to assess cell state dynamics, in response to various compounds and drug candidates, using flow cytometry analysis.
• Conduct a wide range of molecular biology techniques, such as DNA/RNA extraction, PCR, qPCR, cloning, western blotting and chromatin immunoprecipitation (ChiP).
• Interpret, present, and document experiments based on generated data to investigators
• Maintain and report accurate records of data
• Experience with genomics techniques such as ATAC-seq, RNA-seq, ChiP-seq, CUT&RUN is preferred.
• Experience within vivo preclinical models of oncology is also preferred.

• PhD in a relevant field (e.g., cancer biology, molecular biology, genetics, or related disciplines).
• Proficiency in a wide range of molecular biology techniques, including DNA/RNA extraction, PCR, qPCR, cloning, western blotting, chromatin immunoprecipitation (ChiP) and CUT&RUN.
• Proficiency in mammalian cell cultures—particularly organoids.
• Proficiency in bioinformatic analysis.
• Excellent organizational skills and attention to detail.
• Ability to work independently as well as collaboratively in a team-oriented environment.
• Effective communication and interpersonal skills.