Anaerobic Biochemist

NeoplantsAnaerobic BiochemistJob summaryPermanent contractSaint-Ouen-sur-SeineOccasional remoteSalary: Not specifiedStarting date: May 31, 2026Experience: > 5 yearsEducation: PhD or moreSkills & expertiseAttention to detailAutonomyMotivational skillsTeam buildingEnzymeApplySave2 days agoShareNeoplantsInterested in this job?ApplySaveQuestions and answers about the jobIs it mandatory to attach a resume?Does this position allow remote work? What type of contract is offered for this position?Is a cover letter mandatory to apply for this position?What is the start date of the contract?The positionJob descriptionWho we are?Neoplants is a biotech start-up, using cutting-edge synthetic biology techniques to develop the next generation of plants for the benefit of humans and our planet. We have set up a brand-new lab located in Saint-Ouen, where our R&D team is turning incredible visions into real-world products.Working with us means interacting with our scientists in biochemistry, molecular biology, plant transgenesis, genome editing and microbiome enhancement. You will get to exercise your creative thinking, team spirit and leadership capabilities by experiencing work in an ultra fast-moving environment. You will also help bring new ideas and a fresh perspective to our projects.If you are as excited as we are about using state of the art technologies, exploring uncharted scientific territories, pushing the boundaries of what is conceivable, if you want to transform the way people think about genetics and work on inspiring deep-tech products that have a real positive impact on the environment and people’s lives, you’re in the right place.About this roleContext:Neoplants is seeking a highly skilled and motivated scientist to join our team focused on engineering biological nitrogen fixation pathways into plants and bacteria as well as optimizing nitrogenase activity. This role is pivotal for driving innovation in synthetic biology, protein expression / purification, and biochemical characterization of complex enzyme systems, particularly the nitrogenase enzyme.Objective:To lead and execute research projects focused on the expression, purification, characterization, and optimization of nitrogenase and associated components in heterologous systems (E. coli) and for subsequent deployment in plant systems. This includes developing and performing robust anaerobic biochemical assays.Main missions (but not limited to):Design and optimize genetic constructs for heterologous expression of nitrogenase components and accessory proteins in microbial hosts (E. coli).Develop and implement protocols for strict anaerobic growth and manipulation of relevant microbial strains.Establish, optimize, and perform in vitro anaerobic activity assays (ammonia production) for nitrogenase components.Purify and characterize nitrogenase proteins and accessory factors, ensuring structural integrity and functionality under anaerobic conditions.Assess nitrogenase activity and expression in plant systems and engineered microbial co-cultures.Document experimental protocols, results, and data analyses in compliance with GLP, biosafety, and intellectual property guidelines.Become the go to person for setting anaerobic techniques and biochemical assays.Contribute to cross-functional projects by providing technical expertise in protein biochemistry and anaerobic enzymology.Key Techniques:Strict anaerobic microbiology techniques and manipulation ( glove box, anaerobic chambers).High-yield protein expression (e.g., E. coli) and multi-step protein purification (Akta system).Development and execution of quantitative anaerobic enzyme activity assays (Ammonia detection).Detailed biochemical and biophysical characterization of purified proteins (SDS-PAGE, Western blot, spectroscopy).Genetic editing tools (CRISPR/Cas) for microbial strain engineering.Yeast mitochondrial transformation and recombinant protein expression.Why join us?🌿 Contribute to global innovation by collaborating...